The scientists decided to test the five-second rule using watermelon, gummy candy, plain bread, and buttered bread. They chose those foods because each contains a different amount of moisture. “We know water is very important in allowing disease-causing bacteria to transfer,” says Donald Schaffner. He’s the food scientist at Rutgers who led the study.
Next, Schaffner’s team picked four common household surfaces to drop the foods on: tile, stainless steel, wood, and carpet. They spread millions of bacteria onto each test surface.
The final variable, or factor the scientists changed in the experiment, was time. They left the foods on each surface for less than 1 second, for 5 seconds, for 30 seconds, and for 300 seconds.
To make sure their results were reliable, the scientists tried each combination many times. After each trial, they tested the foods to see how much bacteria they had picked up. Would the five-second rule work?